STAT3-Luc/HEK293
CBPB0002
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| I. Background | |
| 癌癥相關(guān)炎癥的特征是存在特定的炎癥細(xì)胞和炎癥介質(zhì)，包括細(xì)胞因子和趨化因子。 最近的證據(jù)表明，信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子（STAT）家族蛋白，特別是 STAT3，在惡性轉(zhuǎn) 化開(kāi)始和癌癥進(jìn)展過(guò)程中，在選擇性誘導(dǎo)和維持癌前炎癥微環(huán)境中起著至關(guān)重要的作用。 信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子 3（STAT3）是一種轉(zhuǎn)錄激活因子，它將細(xì)胞外受體的信號(hào) 直接傳遞到細(xì)胞核，介導(dǎo)一些基因的表達(dá)，在細(xì)胞凋亡等細(xì)胞過(guò)程中發(fā)揮重要作用。在細(xì) 胞因子和生長(zhǎng)因子的作用下，STAT3 被受體相關(guān) Janus 激酶（JAK）磷酸化，形成同二聚 體或異二聚體，并轉(zhuǎn)位到細(xì)胞核中，作為轉(zhuǎn)錄激活劑發(fā)揮作用。STAT3 信號(hào)傳導(dǎo)是惡性腫 瘤的主要內(nèi)在途徑，因?yàn)樗趷盒约?xì)胞中頻繁激活，并且在調(diào)節(jié)腫瘤微環(huán)境中對(duì)癌癥發(fā)炎 至關(guān)重要的許多基因中起關(guān)鍵作用。 |
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| II. Description | |
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STAT3-Luc HEK293 報(bào)告基因細(xì)胞，是由 STAT3 調(diào)控并表達(dá) Luc 熒光素 酶報(bào)告基因的 HEK293 細(xì)胞。IL6 細(xì)胞因子與受體復(fù)合物結(jié)合，啟動(dòng) JAK/STAT3 信號(hào)通路，原理見(jiàn)下圖所示。
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| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | STAT3-Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Acute-Phase Response Factor, DNA-Binding Protein APRF, DNA, ADMIO, Signal Transducer And Activator Of Transcription 3, TATA |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10%FBS+100 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
IV. Description of Host Cell Line |
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| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
Ⅴ. Representative Data |
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Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Human IL-6 Protein in STAT3-Luc HEK293 Cell(C7).  |
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Figure 3. Inhibition of IL-6-induced Reporter Activity by IL-6 Neutralizing Antibody in STAT3-Luc HEK293(C7). |
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